For the phenotypic analysis (primary screen) of your mutant mouse line you have to provide 20 males and 20 females (mutants) and 20 males and 20 females (wildtype). The wildtype animals have to be littermates of the mutant animals. The animals have to be sent when they are 7 weeks old and genotyped. The maximum age difference between youngest and oldest is 7 days. If the groups don't have the appropriate number of animals, the import of mice will be rejected. The Mouse Provider shall keep us informed about the progress of breeding and the preparations for shipping (e.g. start of breeding, birth dates).
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| 1. Please fill in
the request form completely |
| 2. Please wait until
we contact you |
| 3. We might ask for
additional information that should be sent separately by mail
or email: |
| a. |
a health report according to the FELASA rules (example (pdf-file 32 kB)) plus the result of a test for murine Norovirus (MNV) |
| b. |
a complete list of publications concerning
the mouse strain/mutation |
| c. |
a copy of the 3 most important papers |
d.
|
a summary of what is already known about
the mouse strain/mutation (doc-file; templateNov2009; please write in english)
including following areas. This summary will also be used to decide
if findings of phenotypic analysis are "new"
findings. |
| |
| i. |
The role of the gene |
| ii. |
pathways involved |
| iii. |
generation of the mutation, background, breeding
strategy |
| iv. |
features/phenotypes of the mutant mouse strain |
| v. |
possible disease models |
| vi. |
what phenotypes/functions of the gene do you
expect? |
vii.
|
specific conditions: e.g. special
food, cage conditions, known susceptibility because
of the mutation or background (e.g. narcosis) |
|
|
| 4. After receiving
all documents you will be contacted by e-mail if we can consider
your request. |
| 5. When your request is accepted, we arrange a shipping date
according to the provided shipping information and the availability
of free time slots (the mice should be shipped at the age of 7 weeks). We will have to sign a collaboration
agreement (template; pdf-file; 27 kb). |
6. If you think the procedure is not applicable to your mouse line please contact us as soon as possible for further support. |
7. Two weeks before the start of
the breeding: confirmation of the number of breeding pairs
available. |
8. Mate the appropriate
number of animals ( e.g. for heterozygous x heterozygous
breeding with average litter size and fertility we recommend
using at least 40 breeding pairs). Please mate on Tuesday of the week we will announce and continue mating for 7 days, and after the
mice are born, please let us know the number, sex, and date
of birth of the mice (mouse
husbandry; pdf-file; 38 kb). |
| 9. When the mice are 4 weeks old please let us know the number,
sex and genotype of the animals to be sent (a file containing
mouse and parent information has to be supplied with the mice
(a fill-in form for the requested
shipping information (excel sheet; 7 kb) which has to
include the genotype of the mice). Please use our
system for earmarks. We do not accept metal earclips. |
10. Organize shipment of the mice
in the calendar week we announced to you. If possible, the
mice should arrive on tuesday or wednesday of this week.
Arrival time of the mice should not be later than 3:00 pm.
As soon as a forwarder has been assigned, please provide
us with the date and time of arrival as well as the phone
number of the forwarder. Animals should be sent exactly
in the same group in which they were housed in the facility
of origin (specially males originating from different cages
should not be mingled; it is rather likely that they will
fight and hurt each other).
(download: shipment
instructions; pdf-file; 37 kb). |
| 11. Please provide
a scheme of your breeding, starting from the generation of
the mutant mouse line. |
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