clinical chemistry screen

Head

Prof. Dr. Eckhard Wolf

phone : +49 (089) 2180-76800
fax: +49 (089) 2180-76849
e-mail: ewolf@lmb.uni-muenchen.de

Mailing adresses

Institute of Molecular Animal Breeding and Biotechnology
LMU Munich
Feodor-Lynen-Strasse 25
81377 Munich

Helmholtz Zentrum München
German Research Center for Environmental Health
Institute of Experimental Genetics
Ingolstaedter Landstrasse 1
85764 Neuherberg

Scientist

Dr. Birgit Rathkolb

phone : +49 (089) 3187-3282
e-mail: birgit.rathkolb@helmholtz-muenchen.de

Homepage

http://www.gen.vetmed.uni-muenchen.de/index.html

Clinical Chemistry

The aim of the Clinical-Chemical Screen is the detection of hematological changes, defects of various organ systems, and changes in metabolic pathways and electrolyte homeostasis by means of suitable laboratory diagnostic tools. The methods used are routine procedures, allowing the appropriate screen of large numbers of mice for a broad spectrum of clinical-chemical and hematological parameters.

Primary Screen

Pipeline I: Intraperitoneal Glucose Tolerance Test

A glucose tolerance test using a simplified protocol is performed in collaboration with the Metabolic Screen to detect subclinical diabetes and impaired glucose tolerance.

Pipeline II: Clinical-Chemical and Haematological Investigations

Required quantity: At least 130µl plasma in 1.5ml Eppendorf-tubes and 50µl blood in EDTA-coated tubes are required

AU400 - Clinical Chemical Parameters: Sodium, Potassium, Chloride, Calcium, Inorganic Phosphorus, Iron, Total Protein, Albumin, Creatinine, Urea, Cholesterol, Triglycerides, nonesterified fatty acids (NEFA), Glucose, a-Amylase, Aspartat-Aminotransferase (ASAT/GOT), Alanine-Aminotransferase (ALAT/GPT), Alkaline Phosphatase (AP), Lactate-Dehydrogenase (LDH)

ABC Animal Blood Counter - Basic Hematological Parameters:
red blood cell count, white blood cell count, platelet count, hemoglobin, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red cell distribution width (RDW), mean platelet volume (MPV)

Secondary Screen

Depending on the results of the Primary Screen organ- or pathway-specific results will be re-checked and completed by additional parameters which have not been tested in the primary screen. We offer the following profiles for specific screens. Each profile requires a minimum of 90µl Plasma:

Liver-profile
Anaemia-profile (additionally 50µl EDTA-blood needed)
Kidney-profile
Mineral metabolism, skeleton and muscle profile
Measurements of urine samples

The following parameters which can be tested with the AU400 as well are not included in the primary screen:

In Plasma/Serum:

Magnesium, Bicarbonate, Creatine Kinase (CK), Lipase, HDL-Cholesterol, LDL-Cholesterol, Ferritin, Transferrin, Unsaturated Iron Binding Capacity (UIBC), C Reactive Protein (CRP), Total Bilirubin, Direct Bilirubin

In Urine:

Specific Weight, Sodium, Potassium, Chloride, Calcium, Inorganic Phosphate, Total Protein, Albumin, Creatinine, Urea, Uric Acid, Glucose, α-Amylase

If the Primary Screen provides interesting results the following in-depth analysis can be offered:

Total Blood Clotting Time
Serum Insulin Concentration (ELISA)
Insulin-like Growth Factor I (IGF-I, ELISA)
IGF Binding Proteins (Western Blot)
Blood Gas Analysis
Differential Blood Cell Count & Reticulocyte Count
Urinary Protein Electrophoresis

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