| Head |
| Prof. Dr. Andreas Zimmer |
phone : +49 (228) 688 53 03
fax : +49 (228) 688 53 01
e-mail: neuro@uni-bonn.de |
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| Mailing
adress |
Institute of Molecular Psychiatry
University of Bonn
Sigmund Freud Str. 25
53127 Bonn |
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| Screener |
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| Homepage |
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| The pain screen includes
assays for acute thermal, chemical and mechanical as well as
changes in nociceptive thresholds after inflammation. |
| Base
examination (minimum 20 wild type and 20 mutant animals required) |
- Spinal pain reflexes (Tail flick test - Intensity and physical
stimulus (water bath and light beam) are variable.
- Supraspinal pain reaction (hot plate test - end points (first
sign of pain, jumping) and temperature are variable.
- Visceral pain reaction (chemical stimuli (magnesium sulfate
or acetic acid) are used).
- Tonic pain reaction (formaldehyde test - an "early phase"
and a "late phase" is measured as well as the size
of the edema; formaldehyde concentration is variable).
- Carrageenin induced hyperalgesia and allodynia - the thermal
hyperalgesia (hot plate or Hargreaves'), the mechanical
allodynia ("von Frey" test) and
the size of the edema are measured.
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| Secondary
screen (at least 10 test animals and 10 controls per paradigm). |
- Stress induced analgesia (SIA) - an
ancrease in pain latency in the hot plate test is measured.
- Opioid-dependent SIS (swimming stress,
4 min., 30° C)
- Opioid-independent SIA (swimming
stress, 60 sec., 10° C)
- Neuropathological pain models
a lowering of the pain latencies in the hot plate
and/or "von Frey" tests is measured after nerve
ligation.
- Effects of analgesic drugs
(Cox-inhibitors, opioids, cannabinoids, local
anesthetics, nicotine etc.), measured in the hot plate test
and the formaldehyde test.
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