pathology screen

Head:

PD Dr. Irene Esposito

phone : +49 (089) 3187-2636
fax: +49 (089) 3187-3360
e-mail: irene.esposito@helmholtz-muenchen.de

Mailing adress:

Helmholtz Zentrum München - German Research Center for Environmental Health
Institute of Experimental Genetics
Ingolstaedter Landstr. 1
85764 Neuherberg

Scientists:

Dr. Julia Calzada-Wack MD	phone: +49 (089) 3187-2313 fax: +49 (089) 3187-3360 e-mail: calzada@helmholtz-muenchen.de
Dr. Patricia Da Silva-Buttkus	phone: +49 (089) 3187-3241 fax: +49 (089) 3187-3360 e-mail: dasilva-buttkus@helmholtz-muenchen.de
Monica Tost	phone: +49 (089) 3187-3241 fax: +49 (089) 3187-3360 e-mail: monica.tost@helmholtz-muenchen.de

Homepage:

http://www.helmholtz-muenchen.de/en/path/path-home/index.html

http://www.path.med.tu-muenchen.de/

The Pathology screen is a unique institution in Germany that offers many years of experience with mouse pathology under the direction of a highly qualified human pathologist. This combination allows the application of all modern ancillary techniques, used for human pathology, to be applied to the interpretation of mouse models, which aim to reproduce and understand human diseases.

Primary Screen

Mice received in the laboratory of the pathology screen are sacrificed with CO 2. A complete macroscopic examination is performed, including measurement of body weight. S kin, cervical lymph nodes, thymus, spleen, trachea, lung, heart, salivary glands, esophagus, stomach, intestine, liver, pancreatic gland, kidney, urinary bladder, genital organs, brain, cerebellum, adrenal glands, thyroid gland, and parathyroid gland are fixed in 4 % buffered formalin and embedded in paraffin for histological examination, using hematoxylin-eosin stain (H&E).

On request, special stains, immunohistochemistry, X-ray analysis, electron microscopy, FISH, microdissection followed by RT-PCR or PCR analysis, fragmental analysis, Western Blot, and immunoprecipitation could be offered.

Macroscopy

Digital documentation of macroscopic findings

Macroscopy:

Analysis of mice and rats
Complete dissection
Digital documentation

Testis, seminiferous tubules, H&E

Microscopy

Digital documentation of microscopic findings

Microscopy:

Complete histological analysis (H&E stain) of all organs, including bone marrow

Digital documentation (microscopy)

Examples of special stains:

Masson trichrome
Prussian blue
Periodic Schiff’s reagent (PAS)
Van Gieson
Ziehl-Neelson
Giemsa
Gram
Luxol fast blue
Sudan black

steatosis of the liver

Steatosis of the liver H&E

knee, Masson trichrome

Immunohistochemistry (selection of established primary antibodies for mouse, rat, or human tissue):

Ack1	Chk2	MDM2
ALK/p80	cited1	Met
ALK/p80	c-myc	MPO
B220/CD45R	cyclin A	neurofilament protein
Bax	cyclin B1	OB-cadherin
BCL-2	cyclin D1	p14ARF
BCL-6	cyclin D3	p16 INK4A
BCL-10	cyclin E	p21/Kip1
BCL-x S/L	cytoceratin	p27
Calretinin	cytokeratin, pan	wild-type human p53
caspase 3	E2F-1	patched
caspase 3 cleaved	E-cadherin Lul	Pax-5
CD3	E-cadherin	Pax-9
CD5	fast skeletal myosin	p-glycoprotein
CD10	FGFR3	prolactin
CD20cy	FSH beta	RB
CD23	gamma tubulin	S100
CD30	GFAP	Shh
CD31	GH	Skp2 p45
CD43	GLUT-1	Smad1
CD45	HLA-DR	Stat-3
CD56	JAB 1	Stat-3-P
CD79 α cy	kappa light chains	T-box 18
CD117	ki67	TIA-1
CD138	lambda light chains	TER-119
cdk2	lutropin	Tdt
cdk2	lysozyme EC 3.2.1.17	TSH beta
cdk4	Mac-3	von Willebrand
C/EBP ß	Macrophage/L1	ZAP-70
c-erbB-2	MaKL1
Chk1	lL-1

Cerrebellum

Cerebellum, Luxol Fast Blue

Kidney, glomerulus, semifine

Kidney, podocyte’s foot processes

IHC stains are performed with an automated immunostainer

X-ray:

Conventional X-ray

Digital X-ray

Electronmicroscopy

Transmission electron microscope, Zeiss

Electronmicroscopy:

Scanning electron microscopy
(SEM)
Transmission electron microscopy (TEM)

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