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Allergy screens

Introduction

The Allergy screen of the German Mouse Clinic (GMC) identifies allergy or autoimmune-prone mutants to establish mouse models for autoimmune, allergy or skin diseases with defined genotypes and to develop new therapeutic and diagnostic strategies against such diseases.

Primary Screens

  • Total Immunoglobulin plasma Isotype IgE
  • TEWL (TransEpidermal Water Loss )
    Indicator of the skin's barrier function
  • Body Surface Temperature
    Overall indicator marker of animal health (changes in temperature correlate with severity of disease in many murine models)

Secondary Screen

  • Total Immunoglobulin plasma Isotype
    IgA, IgM, IgG1, IgG2a, IgG2b, IgG3 and IgE
  • OVA specific Immunoglobulin plasma isotypes
    IgG and IgE
  • Multiplex measurement of proinflammatory cytokines
    IFN-γ, IL-10, IL-12p70, IL-1β, IL-2, IL-4, IL-5, IL-6, KC/GRO, TNF-α
  • Immnunophenotyping of Leukocytes in bronchoalveolar lavage fluid (BALF) by flow cytometry
  • Inflammation scoring from lung by histology staining
  • Lymphocyte In vitro proliferation assays
  • in vitro Immunoglobulin class switch test

T and B cell lymphocyte functional assays

Ideally, 6 mutant and 6 wild-type control spleens are tested (cell differentiation, Proliferation, cytokine production between other is scoring under a high-throughput in vitro assay).

Airway inflammation model

Ideally, 12 mutant and 12 wild-type control mice are tested in high-throughput screens that include a model of allergic sensitization and aerosol challenge with different optional allergens. Phenotype analyses are based on the rapid quantification of immunoglobulins IgA, IgM, IgG1, IgG2a, IgG2b, IgG3 and IgE from plasma by MSD technology, and of inflammatory cells from bronchoalveolar lavage (BAL) by multi-color flow-cytometry (eosinophils, alveolar macrophages, neutrophils, T, B and NK cells). In addition, immune phenotyping of BAL lymphocytes for the evaluation of allergen-specific T cell responses and a single-step multiplexed quantification of cytokines from BAL fluid are performed.

Models in development stage:

  • Mouse models of autoimmune diseases;
  • Mouse models of systemic anaphylaxis; 
  • Mouse models of skin disease

Head

Dr. rer. nat. Juan Antonio Aguilar-Pimentel   

Tel. Office +49 89 3187-4843
Tel. Lab +49 89 3187-3656
Fax +49 89 3187-2540
E-mail

Staff

Julia Wittmann
Tel. +49 89 3187-3656
Fax +49 89 3187-3661
E-mail

Barbara Weber
Tel. +49 89 3187-3655
E-mail

Expert partner

Prof. Dr. med. Markus Ollert
Department of Infection and Immunity, Luxembourg Institute of Health, Esch-sur-Alzette, Luxembourg  
Department of Dermatology and Allergy Center, Odense Research Center for Anaphylaxis, University of Southern Denmark, Odense, Denmark

Prof. Dr. rer. nat.  Carsten B. Schmidt-Weber Center of Allergy & Environment (ZAUM)Technische Universität München (TUM) and Helmholtz Center Munich