Steroid metabolism (optional)

Introduction

Steroids control the differentiation and proliferation processes of cells and tissues. They participate in the regulation of apoptosis, bone remodelling and neuroregeneration. Severe diseases are caused by monogenic mutations with loss of function of steroid pathway proteins. But defects in steroid metabolism contribute as well to the pathogenesis of many different multifactorial diseases like cancer, diseases of cartilage and bone or neurological diseases. The main focus of the steroid screen is the identification of new animal models for human steroid-related diseases therewith supporting the development of their future medical treatment.

Steroid metabolism parameters:

Primary Screen

  • The key steroids corticosterone, testosterone, and androstenedione will be quantified from mouse plasma by high-throughput LC-MS/MS technology. This validated method has been published in Nature Protocols.


Secondary Screen

 

Further Tests on Demand
 
For very interesting studies we can further quantify dehydroepiandrosterone (DHEA) in mouse plasma using competitive ELISA technique in combination with a special purification protocol.


Head

Dr. Cornelia Prehn    
phone : +49 (089) 3187-3231
fax:      +49 (089) 3187-3225
e-mail:

Expert Partner

Prof. Dr. Jerzy Adamski    
Genome Analysis Center
Institute of Experimental Genetics
Helmholtz Center Munich