Clinical chemistry

Introduction

The aim of the Clinical-Chemical Screen is the detection of hematological changes, defects of various organ systems, and changes in metabolic pathways and electrolyte homeostasis by means of suitable laboratory diagnostic tools. The methods used are routine procedures, allowing the appropriate screen of large numbers of mice for a broad spectrum of clinical-chemical and hematological parameters.


 

Primary Screen

Intraperitoneal Glucose Tolerance Test (IpGTT)

A glucose tolerance test is performed in collaboration with the Energy Metabolism Screen to detect differences in glucose uptake and clearance, indicating for example subclinical diabetes and impaired glucose tolerance.
Clinical-Chemical and Haematological Investigations

Required quantity: At least 90µl Li-heparin plasma in 1.5ml Eppendorf-tubes and 50µl EDTA-blood diluted 1:5 in Sysmex capillary tubes are required

  • AU480 (Beckman-Coulter) - Clinical Chemical Parameters: Sodium, Potassium, Chloride, Calcium, Inorganic Phosphorus, Iron, Unsaturated iron binding capacity (UIBC), Total Protein, Albumin, Creatinine (enzymatic method), Urea, Cholesterol, Triglyceride, Glucose, alpha-Amylase, Aspartat-Aminotransferase (ASAT/GOT), Alanine-Aminotransferase (ALAT/GPT), Alkaline Phosphatase (AP), Lactate-Dehydrogenase (LDH), Fructosamine, measured in 1:2 diluted samples. Additionally calculated: Total iron binding capacity (TIBC) and transferrin saturation,
  • Fasting clinical chemical parameters determined overnight food deprivation: Cholesterol, HDL-Cholesterol, Triglyceride, Non-esterified fatty acids (NEFA), Glycerol, calculated: non-HDL-Cholesterol
  • Sysmex XT2000iV - Basic Hematological Parameters:
    red blood cell count (RBC), white blood cell count (WBC), platelet count (PLT), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red cell distribution width (RDW), mean platelet volume (MPV), platelet distribution width (PDW), platelet large cell ration (P-LCR), plateletcrit (PCT); optionally the complete differential white blood cell count and reticulocyte count and reticulocyte maturity stages can be included.

Primary Screen IMPC

The primary screen of IMPC lines includes the IpGTT and clinical chemistry and hematology analyses of samples collected from ad libitum fed mice during final blood withdrawal:

 

  • AU480 (Beckman-Coulter) - Clinical Chemistry parameters: Sodium, Potassium, Chloride, Calcium, Inorganic Phosphorus, Iron, Unsaturated iron binding capacity (UIBC), Total Bilirubin, Total Protein, Albumin, Creatinine (enzymatic method), Urea, Cholesterol, HDL-cholesterol, Triglyceride, Glucose, alpha-Amylase, Aspartat-Aminotransferase (ASAT/GOT), Alanine-Aminotransferase (ALAT/GPT), Alkaline Phosphatase (AP), Fructosamine measured in undiluted Li-heparin plasma samples
  • Sysmex XT2000iV - Basic Hematological Parameters:
    red blood cell count (RBC), white blood cell count (WBC), platelet count (PLT), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red cell distribution width (RDW), mean platelet volume (MPV), platelet distribution width (PDW), platelet large cell ration (P-LCR), plateletcrit (PCT); optionally the complete differential white blood cell count and reticulocyte count and reticulocyte maturity stages can be included.

Secondary Screen

The following parameters which can be tested with the AU480 as well are not included in the primary screen:

In Plasma/Serum:

Magnesium, Bicarbonate, Creatine Kinase (CK), Lipase, LDL-Cholesterol, Ferritin, Transferrin, C Reactive Protein (CRP), Direct Bilirubin, Uric Acid

In Urine:

Sodium, Potassium, Chloride, Calcium, Inorganic Phosphate, Magnesium, Total Protein, Albumin, Creatinine, Urea, Uric Acid, Glucose

Additional Tests using other devices:

  • Detailed analysis of kidney function using metabolic cages
  • Serum and urine pH and osmolality
  • Plasma levels of hormones (ELISA, MSD-Kit): Insulin, Leptin, T3 and T4, PTH, FGF23, EPO


   

Head

Dr. Birgit Rathkolb    
phone : +49 (089) 3187-3282
e-mail:

Expert Partner

Prof. Dr. Eckhard Wolf    
Chair for Molecular Animal Breeding and Biotechnology
Ludwig-Maximilians-Universität München