Ultimate goal of the Immunology Screen in the German Mouse Clinic is to identify mouse mutant lines, which can serve as representative models for specific human immunological diseases (chronic inflammatory diseases, autoimmune diseases, immune-deficiencies, defects in protective immunity).
The primary screen is based on the analyses of peripheral blood, and blood plasma.
Mainly three methods are used to specify the patterns of leukocyte subsets and immunoglobulines in the examined mice.
- Flow cytometry:
We determine the frequencies of main leukocyte subsets: CD4+, CD8+ T cells, CD4+C25+ T cells, and gamma-delta T cells, B cells, granulocytes, NK cells, NK T cells and monocytes, and measure the frequency of further subsets defined by the expression of CD44, CD62L and Ly6C ( on T cells), IgD and MHC class II (on B cells), and CD11b (on NK cells).
Our platform allows the measurement of the levels of the immunoglobulin isotypes: IgG1, IgG2a, IgG2b, IgG3, IgA, IgM
The occurrence of auto-antibodies is measured by determining the levels of anti-DNA antibodies, and anti-immunoglobulin antibodies (=rheumatoid factor)
Secondary Screen (3 animals per sex/per genotype)
Detailed flow cytometry analysis of the cellular composition of spleen, lymph nodes, bone marrow, thymus.
Antigen-specific immune responses and protective immunity
(only for specific questions, test systems are still in the process of establishment and require a special animal experiment permit)
- antigen-specific antibody response after immunization (Ag-specific ELISA)
- antigen-specific CTL response after immunization (MHC-multimer staining)
- in vivo differentiation of antigen-specific cell populations after immunization (intracellular cytokine staining)
- establishment of antigen-specific protective immunity (Listeria monocytogenes)
phone: +49 (089) 3187-3283
fax: +49 (089) 3187-3500
Prof. Dr. med. Dirk H. Busch
Medical Microbiology, Immunology and Hygiene
Technische Universität München